Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing cytoplasmic staining of macrophages at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of HoxA5 expression in non-transfected 293T: (A), human HoxA5 transfected 293T: (B) and SK-N-SH (C) whole cell lysates.Application
Western blot analysis of HoxA5 expression in non-transfected: (A) and human HoxA5 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of HoxA5 expression in WI 38 whole cell lysate.Application
Western blot analysis of HoxA5 expression in non-transfected: (A) and mouse HoxA5 transfected: (B) 293T whole cell lysates.| Product Name | HoxA5 Antibody (H-125) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 56-180 mapping within an internal region of HoxA5 of human origin |
| Modification Notes | HoxA5 (previously identified as Hox-1.3) is a transcriptional regulator of multiple target genes, including p53 and the progesterone receptor. It is a potent transactivator of p53 and may affect the response of breast cancer cells to DNA damage. In primary breast carcinomas, loss of p53 expression is coupled with loss of HoxA5 expression, suggesting that the loss of HoxA5 expression is important in tumorigenesis. HoxA5 is dynamically expressed during gut development and organogenesis of the respiratory tract, and is continuously expressed from the neonatal period into adult stages in cerebellar Purkinje cells. Expression of HoxA5 is necessary for the region-specific differentiation of the endoderm and differentiation of the myeloid pathway. HoxA5 is also essential for correct specification of the cervical and upper thoracic region of the skeleton and for proper patterning of the embryo. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of HoxA5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 56-180 mapping within an internal region of HoxA5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 56-180 mapping within an internal region of HoxA5 of human origin |
| Entrez Gene | 3202 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing cytoplasmic staining of macrophages at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of HoxA5 expression in non-transfected 293T: (A), human HoxA5 transfected 293T: (B) and SK-N-SH (C) whole cell lysates.
Application
Western blot analysis of HoxA5 expression in non-transfected: (A) and human HoxA5 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of HoxA5 expression in WI 38 whole cell lysate.
Application
Western blot analysis of HoxA5 expression in non-transfected: (A) and mouse HoxA5 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of HoxA5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
