Application
Western blot analysis of MLH1 expression in non-transfected 293T: (A), human MLH1 transfected 293T: (B) and SW480 (C) whole cell lysates and HeLa nuclear extract (D).Application
Western blot analysis of MLH1 expression in non-transfected: (A) and human MLH1 transfected: (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis cancer tissue showing nuclear and cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of MLH1 expression in SW480 (A) and MM-142 (B) nuclear extracts.Application
Immunofluorescence staining of methanol-fixed SW480 cells showing nuclear localization.| Product Name | MLH1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 457-756 mapping at the C-terminus of MLH1 of human origin |
| Modification Notes | DNA-mismatch repair (MMR) is an essential process in maintaining genetic stability. Lack of a functional DNA-mismatch repair pathway is a common characteristic of several different types of human cancers, either due to an MMR gene mutation or promoter methylation gene silencing. MLH1 is an integral part of the protein complex responsible for mismatch repair and is expressed in lymphocytes, heart, colon, breast, lung, spleen, testis, prostate, thyroid and gall bladder tissues, and is methylated in several ovarian tumors. Loss of MLH1 protein expression is associated with a mutated phenotype, microsatellite instability and a predisposition to cancer. In hereditary nonpolyposis colorectal cancer (HNPCC), an autosomal dominant inherited cancer syndrome that signifies a high risk of colorectal and various other types of cancer, the MLH1 gene exhibits a pathogenic mutation. Certain cancer cell lines, including leukemia CCRF-CEM, colon HCT 116 and KM12, and ovarian cancers SK-OV-3 and IGROV-1, show complete deficiency of MLH1, while MLH1 is expressed in 60% of melanomas, 70% of noninvasive squamous cell carcinomas and 30% of invasive squamous cell carcinomas. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAChIP |
| recommended for detection of MLH1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 457-756 mapping at the C-terminus of MLH1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 457-756 mapping at the C-terminus of MLH1 of human origin |
| Entrez Gene | 4292 |
|---|
Application
Western blot analysis of MLH1 expression in non-transfected 293T: (A), human MLH1 transfected 293T: (B) and SW480 (C) whole cell lysates and HeLa nuclear extract (D).
Application
Western blot analysis of MLH1 expression in non-transfected: (A) and human MLH1 transfected: (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis cancer tissue showing nuclear and cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of MLH1 expression in SW480 (A) and MM-142 (B) nuclear extracts.
Application
Immunofluorescence staining of methanol-fixed SW480 cells showing nuclear localization.| Application Notes | recommended for detection of MLH1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
