Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human rectum tissue showing cytoplasmic staining of glandular cells.| Product Name | Myosin X Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1759-2058 mapping at the C-terminus of Myosin X of human origin |
| Modification Notes | Myosins are molecular motors that move along filamentous Actin and influence cellular movements such as phagocytosis. There are seven classes of myosins in vertebrates, including Myosin II and six unconventional Myosin classes: I, V, VI, VII, IX and X. Myosin X (Myo10 or M10) contains three IQ motifs, a Myosin tail homology 4 (MyTH4) domain, a FERM (band 4.1/Ezrin/ Radixin/Moesin) domain, three Pleckstrin homology domains (which mediate phosphatidylinositol phospholipid signaling) and three PEST sites (which may allow cleavage of the Myosin tail). Myosin X binds F-Actin in an ATP-sensitive manner and can influence normal phagocytosis through PI-3 kinase-dependent pathways. Myosin X in cultured cells localizes to the edges of lamellipodia, membrane ruffles and the tips of filopodial Actin bundles. The human Myosin X gene maps to chromosome 5p15.1-p14.3 and encodes a 2,058 amino acid protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Myosin X of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1759-2058 mapping at the C-terminus of Myosin X of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1759-2058 mapping at the C-terminus of Myosin X of human origin |
| Entrez Gene | 4651 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human rectum tissue showing cytoplasmic staining of glandular cells.| Application Notes | recommended for detection of Myosin X of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
