Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic staining of cells in cells in glomeruli at low magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic staining of cells in cells in glomeruli at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Product Name | PIG3 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of PIG3 of human origin |
| Modification Notes | The PIG (p53-induced gene) gene family encodes redox-controlling proteins that are involved in p53 tumor suppressor activity. One member of the PIG gene family, p53-inducible gene 3 (PIG3), is a p53 responsive gene that maps, in humans, to chromosome 2p and encodes a protein with significant homology to oxidoreductases. Oxidoreductases are enzymes involved in cellular responses to oxidative stress and irradiation, and they influence the involvement of PIG3 in the metabolism of reactive oxygen species. PIG3 is localized to the cytoplasm and induced in primary, non-transformed, and transformed cell cultures after exposure to genotoxic agents. The induction of PIG3 is p53 dependent and occurs with delayed kinetics as compared with other p53 downstream targets. PIG3 may act with caspase-8 as a key regulatory element in p53-dependent transcriptional deregulation by triggering the caspase cascade and mitochondrial breakdown. PIG3 is highly up-regulated by p53 and may be useful for detecting transient activation of p53. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PIG3 of human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of PIG3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of PIG3 of human origin |
| Entrez Gene | 9540 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic staining of cells in cells in glomeruli at low magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic staining of cells in cells in glomeruli at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Application Notes | recommended for detection of PIG3 of human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
