Application
Western blot analysis of sEH expression in non-transfected: (A) and mouse sEH transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis cancer tissue showing cytoplasmic staining of tumor cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of sEH expression in mouse liver tissue extract.| Product Name | sEH Antibody (H-215) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Epoxide hydrolase (EHs) are biotransformation enzymes that catalyze the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water. The enzymatic hydration is essentially irreversible and produces mainly metabolites of lower reactivity that can be conjugated and excreted, and, therefore, are generally regarded as detoxifying. Soluble EH (sEH) is a ubiquitous mammalian enzyme for which liver and kidney are reported to have the highest activity. Microsomal EH (mEH) exhibits a broad substrate specificity, while sEH is an enzyme with a "complementary" substrate specificity to mEH. sEH is expressed in 3T3 and HeLa cells. sEH is encoded by the EPHX2 gene, which maps to chromosome 8p21.2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of sEH of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 340-554 of sEH of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 340-554 of sEH of human origin |
| Entrez Gene | 2053 |
|---|
Application
Western blot analysis of sEH expression in non-transfected: (A) and mouse sEH transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis cancer tissue showing cytoplasmic staining of tumor cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of sEH expression in mouse liver tissue extract.| Application Notes | recommended for detection of sEH of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
