Application
Western blot analysis of Grx1 expression in non-transfected: (A) and mouse Grx1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Grx1 expression in non-transfected: (A) and human Grx1 transfected: (B) 293T whole cell lysates.| Product Name | Grx1 Antibody (FL-106) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Glutaredoxin (Grx) and a relative, thioredoxin, catalyze general thiol-disulfide oxidoreductions and act as hydrogen donors for ribonucleotide reductase, an enzyme essential for DNA synthesis. Proteins which catalyze thiol-disulfide exchange reactions are required for electron and proton transport to essential enzymes like ribonucleotide reductase, for the formation of disulfide bonds during protein folding, and for general regulation of protein function by thiol redox control. These proteins also play a role in cellular defense against oxidative stress. The thioredoxin superfamily includes a number of proteins with the same basic folding and structure as thioredoxin and glutaredoxin, with the active site at the C-terminal end of a ∫-strand followed by an å-helix. Glutaredoxin (Grx) operates in thiol-disulfide reactions via two vicinal (CXYC) active site cysteine residues, which either form a disulfide (oxidized form) or a dithiol (reduced form). Mammalian cells contain at least two dithiol glutaredoxins: Grx1, the cytoplasmic form; and Grx2, which has mitochondrial and nuclear isoforms. Nuclear Grx2, unlike Grx1, is a substrate for thioredoxin reductase and has a higher affinity for S-glutathionylated proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Grx1 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with Grx2 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-106 representing full length Grx1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-106 representing full length Grx1 of human origin |
| Entrez Gene | 2745 |
|---|
Application
Western blot analysis of Grx1 expression in non-transfected: (A) and mouse Grx1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Grx1 expression in non-transfected: (A) and human Grx1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Grx1 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with Grx2 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
