| Product Name | AE2 Antibody (H-150) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 100-255 mapping within an N-terminal cytoplasmic domain of AE2 of human origin |
| Modification Notes | Primary canalicular bile undergoes a process of fluidization and alkalinization along the biliary tract that is influenced by several factors, including hormones, innervation/neuropeptides and biliary constituents. The excretion of bicarbonate at both the canaliculi and the bile ducts is an important contributor to the generation of bile-salt independent flow. Bicarbonate is secreted from hepatocytes and cholangiocytes through parallel mechanisms, which involve chloride efflux through activation of chloride channels and further bicarbonate secretion via AE2 (also designated SLC4A2)-mediated chloride/bicarbonate exchange. The AE2 protein regulates pH, chloride concentration, cell volume and transepithelial ion transport in many tissues. Gene silencing of AE2 causes a marked inhibition of unstimulated and secretin-stimulated chloride/bicarbonate exchange, which maintains the bile acid pool that is crucial for secretin to induce bicarbonate-rich choleresis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of AE2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 100-255 mapping within an N-terminal cytoplasmic domain of AE2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 100-255 mapping within an N-terminal cytoplasmic domain of AE2 of human origin |
| Entrez Gene | 6522 |
|---|
| Application Notes | recommended for detection of AE2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
