| Product Name | PI 3-kinase p85α/β/γ Antibody (H-274) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 323-596 mapping within an internal region of PI 3-kinase p85β of human origin |
| Modification Notes | Phosphatidylinositol 3-kinase (PI 3-kinase) phosphorylates the 3' OH position of the inositol ring of inositol lipids and is composed of p85 and p110 subunits. PI 3-kinase p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Both PI 3-kinase p85å and PI 3-kinase p85β possess one SH3 and two SH2 domains. PI 3-kinase p85α, also known as GRB1, phosphatidylinositol 3-kinase regulatory 1 or p85, is a 724 amino acid protein that exists as four alternatively spliced isoforms. Involved in insulin metabolism, defects in the PI 3-kinase p85α gene have been linked to insulin resistance. PI 3-kinase p85α is polyubiquitinated in T cells by Cbl-b, and has multiple phosphorylated amino acid residues, including a phosphorylated tyrosine residue at position 467. PI 3-kinase p85© contains 1,102 amino acids and is encoded by a gene that maps to human chromosome 7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PI 3-kinase p85α, PI 3-kinase p85β, PI 3-kinase p85γ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 323-596 mapping within an internal region of PI 3-kinase p85&beta of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 323-596 mapping within an internal region of PI 3-kinase p85β of human origin |
| Entrez Gene | 5296 |
|---|
| Application Notes | recommended for detection of PI 3-kinase p85α, PI 3-kinase p85β, PI 3-kinase p85γ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
