Application
Western blot analysis of Filamin 3 expression in U-87 MG (A) and NIH/3T3 (B) whole cell lysates.| Product Name | Filamin 3 Antibody (H-65) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 386-450 mapping within an internal region of Filamin 3 of human origin |
| Modification Notes | Filamins are Actin-binding proteins which contain an N-terminal Actin-binding domain, a membrane glycoprotein domain and a C-terminal self-association domain. Filamins help reshape the cytoskeleton by forming flexible cross-links between two Actin filaments, which maintain membrane integrity during force application. Filamins also participate in signal transduction pathways associated with cell motility, adhesion, differentiation and survival, and force transduction. The filamin family is comprised of Filamin 1, Filamin 2 and Filamin 3. Filamin 3, also designated Filamin B and ∫-Filamin, is a form of Filamin that plays a role in endochondral ossification, vertebral segmentation and joint formation. The interaction of Filamin 3 with Filamin 1 may allow neuroblast migration into the cortical plate from the ventricular zone. Muta-tions in the gene that encodes for Filamin 3, FLNB, are associated with five human skeletal disorders, specifically, autosomal dominant Larsen syndrome, spondylocarpotarsal syndrome, type I atelosteogenesis, type III atelosteogenesis and Boomerang dysplasia as well as the neurologic disorder peri-ventricular heterotopia. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Filamin 3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 386-450 mapping within an internal region of Filamin 3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 386-450 mapping within an internal region of Filamin 3 of human origin |
| Entrez Gene | 2317 |
|---|
Application
Western blot analysis of Filamin 3 expression in U-87 MG (A) and NIH/3T3 (B) whole cell lysates.| Application Notes | recommended for detection of Filamin 3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
