Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes.Application
Western blot analysis of Syne-1 expression in SW-13 whole cell lysate (A) and HeLa nuclear extract (B).| Product Name | Syne-1 Antibody (H-100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Synaptic nuclear envelope protein-1 (Syne-1) is a member of the Golgi- and nuclear envelope-localized spectrin family, and it facilitates retrograde vesicular trafficking from the Golgi to the ER. Syne-1 is a 8,797 amino acid protein that contains several spectrin repeats similar to those in dystrophin and utrophin, two tandem calponin homology domains at its N-terminus, as well as a domain that is homologous to the C-terminal of Klarsicht, a Drosophila protein associated with nuclei and necessary for a few nuclear migrations. Syne-1 localizes to the Golgi apparatus and nuclear envelope until cytokinesis, when it migrates to the central spindle and midbody, where it functions together with KIF3B to assist the accumulation of the membrane vesicles at the spindle midbody. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Syne-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 8537-8636 mapping near the C-terminus of Syne-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 8537-8636 mapping near the C-terminus of Syne-1 of human origin |
| Entrez Gene | 23345 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes.
Application
Western blot analysis of Syne-1 expression in SW-13 whole cell lysate (A) and HeLa nuclear extract (B).| Application Notes | recommended for detection of Syne-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
