Application
Western blot analysis of MAP LC3α expression in mouse brain tissue extract.Application
Western blot analysis of MAP LC3α expression in human brain tissue extract in the absence (A) and the presence (B) of immunizing peptide, mouse brain (C) tissue extract and rat brain (D) tissue extract.| Product Name | MAP LC3α Antibody (R-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP-light chain 3 beta (MAP-LC3∫) and MAP-light chain 3 alpha (MAP-LC3å), both of which are mammalian homologs of yeast Apg8, are subunits that can associate with either MAP-1A or MAP-1B. While MAP-LC3∫ is essential for autophagy and is associated with autophagosome membranes after processing, MAP LC3å is involved in the formation of autophagosomal vacuoles and is localized to the intracytoplasmic membrane. MAP LC3å is expressed as two alternatively spliced isoforms that are expressed in testis, brain, heart, liver and skeletal muscle, but are absent in thymus and peripheral blood leukocytes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| See 1 citations for MAP LC3&alphaAntibody (R-23) | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of MAP LC3&alpha of mouse rat human and zebrafish origin by WB IP IF and IHC(P) also reactive with additional species including bovine |
|---|---|
| Tissue Specificity | recommended for detection of MAP LC3α of mouse, rat, human and zebrafish origin by WB, IP, IF and IHC(P); also reactive with additional species, including bovine |
| Entrez Gene | 84557 |
|---|
Application
Western blot analysis of MAP LC3α expression in mouse brain tissue extract.
Application
Western blot analysis of MAP LC3α expression in human brain tissue extract in the absence (A) and the presence (B) of immunizing peptide, mouse brain (C) tissue extract and rat brain (D) tissue extract.| Application Notes | See 1 citations for MAP LC3&alphaAntibody (R-23) |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
