Application
Antibodies tested include p-PDGFR-β (Tyr 579): (A,B,C) and PDGFR-β (11H4): (D,E,F).Application
Western blot analysis of PDGFR-β phosphorylation on untreated (A) and PDGF-B treated (B) Hep G2 cells transfected with PDGFR-β.Application
Western blot analysis of PDGFR-β phosphorylation in non-transfected: (A) and human PDGFR-β transfected: (B) 293T whole cell lysates.| Product Name | p-PDGFR-β Antibody (Tyr 579) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Platelet-derived growth factor (PDGF) is a mitogen for mesenchyme- and glia-derived cells. PDGF consists of two chains, A and B, which dimerize to form functionally distinct isoforms, PGDF-AA, PDGF-AB and PDGF-BB. These three isoforms bind with different affinities to two receptor types, PDGFR-å and -∫, which are endowed with protein tyrosine kinase domains. PDGFR-å can bind to both A and B subunits of PDGF, while PDGFR-∫ can only bind the B subunit. Ligand binding promotes either homo- or heterodimerization of the PDGF receptors in a specific manner. PDGF-AA induces the dimerization of two å receptors, PDGF-AB induces dimerization of åå and å∫ and PDGF-BB induces the formation of three types of dimers, åå, å∫ and ∫∫. Translocation of the PDGFR-∫ gene with the Tel gene is linked to chronic myelomonocytic leukemia (CMML), a myelodysplastic syndrome, and demonstrates the oncogenic potential of the PDGF receptors. Both mouse and human PDGFR-∫ are subject to phosphorylation on specific amino acid residues, such as Tyr 579. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Tyr 579 phosphorylated PDGFR-&beta of mouse and human origin by WB and IP |
|---|---|
| Tissue Specificity | recommended for detection of Tyr 579 phosphorylated PDGFR-β of mouse and human origin by WB and IP |
| Entrez Gene | 5159 |
|---|
Application
Antibodies tested include p-PDGFR-β (Tyr 579): (A,B,C) and PDGFR-β (11H4): (D,E,F).
Application
Western blot analysis of PDGFR-β phosphorylation on untreated (A) and PDGF-B treated (B) Hep G2 cells transfected with PDGFR-β.
Application
Western blot analysis of PDGFR-β phosphorylation in non-transfected: (A) and human PDGFR-β transfected: (B) 293T whole cell lysates.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
