Application
Western blot analysis of JMJD2A expression in non-transfected (A) and human JMJD2A transfected (B) whole cell lysates.| Product Name | JMJD2A Antibody (H-287) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 347-633 mapping within an internal region of JMJD2A of human origin |
| Modification Notes | JMJD2A (jumonji domain containing 2A), also designated jumonji C domain-containing histone demethylation protein 3A, is a 1,064 amino acid protein encoded by the human gene JMJD2A. JMJD2A belongs to the JHDM3 histone demethylase family and contains one JmjC domain, one JmjN domain, two PHD-type zinc fingers and two Tudor domains. JMJD2A is histone de-methylase that specifically demethylates Lys 9 and Lys 36 residues of Histone H3, thereby playing a central role in histone code. It does not demethylate histone H3 Lys 4, H3 Lys 27 nor H4 Lys 20, however, it will demethylate tri-methylated H3 Lys 9 and H3 Lys 36 residue, while it has no activity on mono- and dimethylated residues. JMJD2A demethylation of lysine residues will generate formaldehyde and succinate. It also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. JMJD2A is a ubiquitously expressed nuclear protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of JMJD2A of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 347-633 mapping within an internal region of JMJD2A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 347-633 mapping within an internal region of JMJD2A of human origin |
| Entrez Gene | 9682 |
|---|
Application
Western blot analysis of JMJD2A expression in non-transfected (A) and human JMJD2A transfected (B) whole cell lysates.| Application Notes | recommended for detection of JMJD2A of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
