Application
Western blot analysis of Med4 expression in 293T whole cell lysate.| Product Name | Med4 Antibody (C-16) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | In mammalian cells, transcription is regulated in part by high molecular weight co-activating complexes that mediate signals between transcriptional activators and RNA polymerase II (Pol II). The mediator complex is one such multi-protein structure that functions as a bridge between regulatory proteins and Pol II, thereby regulating Pol II-dependent transcription. Med4 (mediator complex subunit 4), also known as ARC36, DRIP36 or VDRIP, is a 270 amino acid protein that localizes to the nucleus and exists as a component of the mediator complex. Working in tandem with several other proteins, including Med8 and Med25, Med4 serves as a scaffold for the assembly of a functional preinitiation complex with Pol II and general transcription factors, thereby activating the transcription of Pol II-dependent genes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Med4 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with other Med family members also reactive with additional species, including equine, canine, bovine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of Med4 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of Med4 of human origin |
| Entrez Gene | 29079 |
|---|
Application
Western blot analysis of Med4 expression in 293T whole cell lysate.| Application Notes | recommended for detection of Med4 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with other Med family members also reactive with additional species, including equine, canine, bovine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
