Application
Antibodies tested include p-Smad3 (Ser 208): (A, B, C) and Smad2/3 (FL-425): (D, E, F).Application
Western blot analysis of p-Smad3 expression in A2058 tissue extract.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Product Name | p-Smad3 Antibody (Ser 208) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGF∫/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGF∫ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGF∫ signaling by interfering with TGF∫-mediated phosphorylation of other Smad family members. Human Smad3 is subject to phosphorylation by TGF∫ on specific amino acid residues, including Ser 208. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 208 phosphorylated Smad3 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Ser 208 phosphorylated Smad3 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 4088 |
|---|
Application
Antibodies tested include p-Smad3 (Ser 208): (A, B, C) and Smad2/3 (FL-425): (D, E, F).
Application
Western blot analysis of p-Smad3 expression in A2058 tissue extract.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Application Notes | WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
