Application
Western blot analysis of KIAA0020 expression in non-transfected: (A) and human KIAA0020 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of KIAA0020 expression in Hep G2 whole cell lysate.Application
Immuno-peroxidase staining of formalin fixed, paraffin-embedded human lung showing nuclear staining.| Product Name | KIAA0020 Antibody (B-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of KIAA0020 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | KIAA0020, also known as XTP5 (HBV X-transactivated gene 5 protein), PUF6, PEN or HLA-HA8, is a 648 amino acid nuclear protein that contains six pumilio repeats and one PUM-H (pumilio homology) domain. The pumilio repeat is an imperfectly repeated 36 amino acid motif that is flanked by short N- and C-terminal regions which, together, comprise the PUM-H domain. Proteins that contain PUM-H domains usually exhibit sequence-specific RNA binding capabilities and often play a role in repressing the translation of select mRNAs. Expressed ubiquitously with highest expression in liver, kidney, lung, colon, ovary and testis, KIAA0020 contains an HA-8 (histocompatibility antigen-8) region that can be cleaved and exposed at the cell surface, where it may function as a minor histocompatibility antigen. Due to the presence of a PUM-H domain, KIAA0020 may be involved in the regulation of translation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of KIAA0020 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of KIAA0020 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 9933 |
|---|
Application
Western blot analysis of KIAA0020 expression in non-transfected: (A) and human KIAA0020 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of KIAA0020 expression in Hep G2 whole cell lysate.
Application
Immuno-peroxidase staining of formalin fixed, paraffin-embedded human lung showing nuclear staining.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
