Application
Western blot analysis of CYP2F1 expression in mouse lung tissue extract.Application
Western blot analysis of CYP2F1 expression in WI 38 whole cell lysate (A) and rat lung (B) and mouse lung (C) tissue extracts.| Product Name | CYP2F1 Antibody (H-41) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 451-491 mapping at the C-terminus of CYP2F1 of human origin |
| Modification Notes | CYP2F1 (cytochrome P450 2F1) is a human-specific membrane-bound protein usually localized to the endoplasmic reticulum membrane. Cytochromes, which are incredibly polymorphic, generally catalyze redox reactions. CYP2F1 is a lung-specific cytochrome P450 (pigment at 450 nm) that is involved in metabolizing potentially carcinogenic pneumotoxins. Its polymorphic nature likely has an impact on the sensitivity of an individual to such toxins. Specifically within Clara cells, CYP2F1 converts Skatole (3-methylindole) to 3-methyleneindolenine, an electrophile that disrupts cell functions by forming dangerous protein adducts. Skatole has been used to supplement flavor in cigarettes. CYP2F2 is the murine homolog of human CYP2F1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CYP2F1 of human origin and CYP2F2 of mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 451-491 mapping at the C-terminus of CYP2F1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 451-491 mapping at the C-terminus of CYP2F1 of human origin |
| Entrez Gene | 1572 |
|---|
Application
Western blot analysis of CYP2F1 expression in mouse lung tissue extract.
Application
Western blot analysis of CYP2F1 expression in WI 38 whole cell lysate (A) and rat lung (B) and mouse lung (C) tissue extracts.| Application Notes | recommended for detection of CYP2F1 of human origin and CYP2F2 of mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
