Application
Western blot analysis of Tra-2 expression in non-transfected: (A) and human Tra-2 transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).Application
Western blot analysis of Tra-2β expression in HeLa (A) and Hep G2 (B) nuclear extracts.Application
Western blot analysis of Tra-2β expression in HeLa nuclear extract.Application
Western blot analysis of Tra-2β expression in IMR-32 (A), SK-N-MC (B) and HL-60 (C) nuclear extracts.| Product Name | Tra-2β Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-50 mapping at the N-terminus of Tra-2β of human origin |
| Modification Notes | Human transformer-2 alpha (Tra-2å) and Tra-2∫ are nuclear proteins that associate with distinct pre-mRNA splicing enhancer elements. Tra-2å is the functional homolog of the Drosophila TRA-2 protein, which regulates the female specific pre-mRNA splicing pattern of the doublesex (dsx) gene in Drosophila. Human Tra-2 proteins are able to actively splice Drosophila sex factors; however, human Tra-2å has not been shown to induce sexual differentiation suggesting that human Tra-2å may regulate splicing patterns involving alternative mechanisms. Tra-2å and Tra-2∫ contain a single RNP-type RNA-binding domain and selectively bind to purine-rich sequences to facilitate mRNA splicing. Expression of Tra-2∫ is upregulated during the reoxygenation of hypoxic astrocytes. Tra-2å and Tra-2∫ interact with the serine/arginine-rich (SR) family of splicing factors to form Tra-2/SR complexes that then regulate tissue-specific alternative splicing patterns of many pre-mRNAs. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of Tra-2β isoform 1, and to a lesser extent, other splicing factor family members of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-50 mapping at the N-terminus of Tra-2&beta of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-50 mapping at the N-terminus of Tra-2β of human origin |
| Entrez Gene | 6434 |
|---|
Application
Western blot analysis of Tra-2 expression in non-transfected: (A) and human Tra-2 transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).
Application
Western blot analysis of Tra-2β expression in HeLa (A) and Hep G2 (B) nuclear extracts.
Application
Western blot analysis of Tra-2β expression in HeLa nuclear extract.
Application
Western blot analysis of Tra-2β expression in IMR-32 (A), SK-N-MC (B) and HL-60 (C) nuclear extracts.| Application Notes | recommended for detection of Tra-2β isoform 1, and to a lesser extent, other splicing factor family members of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
