Application
Western blot analysis of Ikaros 3 expression in non-transfected: (A) and human Ikaros 3 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Ikaros 3 expression in Hep G2 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing nuclear and cytoplasmic staining.| Product Name | Aiolos Antibody (O-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against Aiolos of human origin |
| Modification Notes | Ikaros family members, including Ikaros and Helios, are nuclear factors that colocalize with DNA replication machinery components in higher-order chromatin structures and respond to signaling events, such as T-cell activation. Helios and Ikaros bind to similar DNA sequences and they function as hemopoietic-specific transcription factors. Members of the Ikaros family contain zinc-finger domains that are involved in DNA-binding and in the formation of homodimers and heterodimers between Ikaros family members. Aiolos, also known as zinc finger protein Ikaros 3 or ZNFN1A3, is a 509 amino acid nuclear protein. Aiolos plays an important role in lymphocyte differentiation regulation and, via this role, mutated Aiolos is implicated in leukemogenesis. Expressed in most tissues, Aiolos is predominantly found in spleen, thymus and peripheral blood leukocytes. Aiolos contains six C2H2-type zinc fingers, a motif commonly involved in nucleotide binding. Aiolos interacts with Ikaros family members, including Eos and Pegasus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Aiolos of human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against Aiolos of human origin |
|---|---|
| Tissue Specificity | raised against Aiolos of human origin |
| Entrez Gene | 22806 |
|---|
Application
Western blot analysis of Ikaros 3 expression in non-transfected: (A) and human Ikaros 3 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Ikaros 3 expression in Hep G2 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing nuclear and cytoplasmic staining.| Application Notes | recommended for detection of Aiolos of human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
