Application
Western blot analysis of CD177 expression in human PBL (A) and CCRF-CEM (B) whole cell lysates.| Product Name | CD177 Antibody (H-221) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Patients with polycythemia vera (PV), a neoplastic stem cell disorder that leads to excessive production of all myeloid cell lines, overexpress the cell surface antigen CD177, also designated NB1. The increased output, especially of red blood cells, increases whole blood viscosity and causes vascular occlusion and ischemia. Because of the marked upregulation of CD177 in PV patients, as compared to healthy individuals or those with other erythrocytosis-related conditions, analysis of this protein presents a useful tool for diagnosis and research into the mechanisms of PV. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CD177 of human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 27-247 mapping within an internal region of CD177 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 27-247 mapping within an internal region of CD177 of human origin |
| Entrez Gene | 57126 |
|---|
Application
Western blot analysis of CD177 expression in human PBL (A) and CCRF-CEM (B) whole cell lysates.| Application Notes | recommended for detection of CD177 of human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
