Application
Western blot analysis of N-WASP expression in non-transfected: (A) and mouse N-WASP transfected: (B) 293T whole cell lysates.Application
Western blot analysis of N-WASP expression in SK-N-SH (A) and SH-SY5Y (B) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic and extracellular staining of glandular cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed SK-N-SH cells showing cytoplasmic localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse brain tissue showing cytoplasmic localization.Application
Western blot analysis of N-WASP expression in SK-N-SH whole cell lysate.| Product Name | N-WASP Antibody (H-100) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The Wiskott-Aldrich syndrome (WAS) is characterized by thrombocytopenia, eczema, defects in cell-mediated and humoral immunity, and a propensity for lymphoproliferative diseases. The syndrome is the result of a mutation in the gene encoding a proline-rich protein termed WASP. WASP and the related protein neural-WASP (or N-WASP) are downstream effectors of Cdc42. Both WASP and N-WASP are implicated in Actin polymerization and cytoskeletal organization, and N-WASP is also essential for mediating the Cdc42-induced formation of filopodia. WASP is primarily expressed in hematopoietic cells, whereas N-WASP is richest in neural tissues and is also expressed ubiquitously. The effects of Cdc42-stimulated Actin assembly require the interaction of WASP/N-WASP with the Arp2/3 complex, which dramatically enhances polymerization. The WASP and N-WASP proteins characteristically contain a pleckstrin homology (PH) domain, which binds phosphatidyl-inositol bisphosphate (PIP2); a Cdc42-binding domain; and a 70 amino acid conserved verprolin-homology (VPH) domain, which is the Actin-binding region and is critical to the regulation of the Actin cytoskeleton. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of N-WASP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 111-210 mapping within an internal region of N-WASP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 111-210 mapping within an internal region of N-WASP of human origin |
| Entrez Gene | 8976 |
|---|
Application
Western blot analysis of N-WASP expression in non-transfected: (A) and mouse N-WASP transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of N-WASP expression in SK-N-SH (A) and SH-SY5Y (B) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic and extracellular staining of glandular cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed SK-N-SH cells showing cytoplasmic localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse brain tissue showing cytoplasmic localization.
Application
Western blot analysis of N-WASP expression in SK-N-SH whole cell lysate.| Application Notes | recommended for detection of N-WASP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
