Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization.Application
Western blot analysis of Rb phosphorylation expression in untreated (A) and UV treated (B) 3T3 whole cell lysates.| Product Name | p-Rb Antibody (Ser 807) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ser 807 phosphorylated Rb of human origin, Ser 800 phosphorylated Rb of mouse origin, and Ser 778 phosphorylated Rb of rat origin of m, r and h origin by WB, IP, IF and IHC(P) |
| Modification | Phosphoralated |
| Modification Notes | Pediatric cancer retinoblastoma and the formation of other human tumors can be attributed to mutations in the retinoblastoma tumor suppressor gene (Rb). The Rb protein regulates differentiation, apoptosis and cell cycle control by coordinating the cell cycle at G1-S with transcriptional machinery. During G1, cyclin D-dependent kinase-mediated phosphorylation of Rb at Ser 795 marks the conversion of Rb from a transcriptionally repressive, hypophosphorylated state to an inactive, phosphorylated state, which may be sustained through mitosis by differential phosphorylation of up to 16 putative serine or threonine residues, including Ser 249/Thr 252, Thr 373, Thr 356, Ser 780, Ser-807/ Ser 811 and Thr 821/Thr 826. Hypophosphorylated Rb represses the transcription of genes controlling the cell cycle through direct protein-protein interactions and through the recruitment of histone deacetylase. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 807 phosphorylated Rb of human origin Ser 800 phosphorylated Rb of mouse origin and Ser 778 phosphorylated Rb of rat origin of m r and h origin by WB IP IF and IHC(P) |
|---|---|
| Tissue Specificity | recommended for detection of Ser 807 phosphorylated Rb of human origin, Ser 800 phosphorylated Rb of mouse origin, and Ser 778 phosphorylated Rb of rat origin of m, r and h origin by WB, IP, IF and IHC(P) |
| Entrez Gene | 5925 |
|---|
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization.
Application
Western blot analysis of Rb phosphorylation expression in untreated (A) and UV treated (B) 3T3 whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
