Application
Western blot analysis of RGS7 expression in non-transfected: (A) and human RGS7 transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic staining of cells in molecular and granular layers and Purkinje cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane and cytoplasmic staining.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of RGS6 and RGS7 expression in TT whole cell lysate (A) and mouse cerebellum tissue extract (B).| Product Name | RGS6/7 Antibody (H-190) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 306-495 (deletion 454-471) mapping at the C-terminus of RGS7 of human origin |
| Modification Notes | Heterotrimeric G proteins function to relay information from cell surface receptors to various intracellular effectors. G proteins comprise å, ß and g subunits, and following activation the å subunit binds GTP and dissociates from the ßg complex (1). A large group of proteins have been identified as GTPase-activating proteins (GAPs), including the RGS (regulator of G protein signaling) family, which serve to deactivate specific Gå isoforms by increasing the rate at which they convert GTP to GDP (2,3). A subfamily of RGS proteins expressed in the central nervous system contain, in addition to the highly conserved RGS domain, a characteristic GGL domain, or G protein g subunit-like domain, which mediates binding to Gß 5 subunits (4,5). This subfamily, which includes RGS6, RGS7, RGS9 and RGS11, associates with Gß 5 to form active GAP complexes that are predominantly localized to the cytosol (6). RGS/ß5 complexes preferentially target Gåo subunit for hydrolysis and inhibit Gß1g2-mediated activation of phospholipase C (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of RGS6 and isoforms 1-4 of RGS7 of mouse, rat and human origin by WB, IP, IF, IHC(P)and ELISA also reactive with additional species, including equine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 306-495 (deletion 454-471) mapping at the C-terminus of RGS7 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 306-495 (deletion 454-471) mapping at the C-terminus of RGS7 of human origin |
| Entrez Gene | 6000 |
|---|
Application
Western blot analysis of RGS7 expression in non-transfected: (A) and human RGS7 transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic staining of cells in molecular and granular layers and Purkinje cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane and cytoplasmic staining.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of RGS6 and RGS7 expression in TT whole cell lysate (A) and mouse cerebellum tissue extract (B).| Application Notes | recommended for detection of RGS6 and isoforms 1-4 of RGS7 of mouse, rat and human origin by WB, IP, IF, IHC(P)and ELISA also reactive with additional species, including equine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
