Application
Western blot analysis of CLIM-2 expression in non-transfected: (A) and human CLIM-2 transfected: (B) 293 whole cell lysates and HeLa nuclear extract (C).Application
Western blot analysis of CLIM-1/2 expression in HeLa nuclear extract.Application
Western blot analysis of CLIM-2 expression in HeLa whole cell lysate.Application
Western blot analysis of CLIM-2 expression in non-transfected 293T: (A), mouse CLIM-2 transfected 293T: (B) and CCRF-CEM (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing nuclear staining of cells in granular and molecular layers and Purkinje cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of CLIM-1 and CLIM-2 expression in CCRF-CEM nuclear extract.| Product Name | CLIM-1/2 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 of CLIM-1 of human origin |
| Modification Notes | CLIM-1/2 (LIM domain-binding factor-2, LDB1,CLP36, ELFIN, CLP-36, enigma homolog) is a LIM domain-containing protein. LIM domain-containing proteins are capable of binding to a variety of transcription factors. They are likely to function as enhancers, to bring together diverse transcription factors and form higher order activation complexes, or to block formation of such complexes. Human CLIM-1 is a cytoplasmic protein. CLIM-1 northern blot analysis reveals expression of an approximately 2.0-kb transcript most abundant in heart and skeletal muscle. CLIM-1 interacts with the C-terminal EF hand region of alpha-actinin-2 through a LIM domain. CLIM-1 localizes to actin stress fibers in nonmuscle cells and associates with alpha-actinin via its PDZ-domain. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of CLIM-1 and CLIM-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 of CLIM-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 of CLIM-1 of human origin |
| Entrez Gene | 8861 |
|---|
Application
Western blot analysis of CLIM-2 expression in non-transfected: (A) and human CLIM-2 transfected: (B) 293 whole cell lysates and HeLa nuclear extract (C).
Application
Western blot analysis of CLIM-1/2 expression in HeLa nuclear extract.
Application
Western blot analysis of CLIM-2 expression in HeLa whole cell lysate.
Application
Western blot analysis of CLIM-2 expression in non-transfected 293T: (A), mouse CLIM-2 transfected 293T: (B) and CCRF-CEM (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing nuclear staining of cells in granular and molecular layers and Purkinje cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of CLIM-1 and CLIM-2 expression in CCRF-CEM nuclear extract.| Application Notes | recommended for detection of CLIM-1 and CLIM-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
