Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing Z discs and cytoplasmic staining of myocytes.Application
Immunofluorescence staining of methanol-fixed K-562 cells showing membrane localization.Application
Western blot analysis of Flt-3/Flk-2 expression in rat brain tissue extract.| Product Name | Flt-3/Flk-2 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 representing full length Flt-3/Flk-2 of human origin |
| Modification Notes | Stem cell tyrosine kinase (STK-1) has been cloned from a CD34+ hematopoietic stem cell enriched library and identified as the human homolog of a previously identified gene of mouse origin designated either Flk-2 or Flt-3. The STK-1 cDNA encodes a protein of 993 amino acids with 85% identity to Flt-3/Flk-2. STK-1 is a member of the type III receptor tyrosine kinase family that includes Kit (steel factor receptor), Fms and PDGF. STK-1 expression in blood and marrow is restricted to CD34+ cells, a population greatly enriched for hematopoietic stem/progenitor cells. STK-1 antiserum recognizes two polypeptides in these cells. The mouse homolog of STK-1, designated Flt-3/Flk-2, is expressed at high levels in hematopoietic cells and also in neural, gonadal, hepatic and placental tissues. It has been suggested that STK-1 and its murine homolog Flt-3/Flk-2 may function as growth factor receptors on hematopoietic stem and/or progenitor cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Flt-3/Flk-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 representing full length Flt-3/Flk-2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 representing full length Flt-3/Flk-2 of human origin |
| Entrez Gene | 2322 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing Z discs and cytoplasmic staining of myocytes.
Application
Immunofluorescence staining of methanol-fixed K-562 cells showing membrane localization.
Application
Western blot analysis of Flt-3/Flk-2 expression in rat brain tissue extract.| Application Notes | recommended for detection of Flt-3/Flk-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
