Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lower stomach tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of MCAD expression in non-transfected 293T: (A), human MCAD transfected 293T: (B) and HeLa (C) whole cell lysates.| Product Name | MCAD Antibody (H-180) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 196-375 mapping within an internal region of MCAD of human origin |
| Modification Notes | Acyl-CoA dehydrogenase is a family of enzymes that localize to the mitochondrion and target acyl chain lengths of 4 to 16 by use of the mitochondrial fatty acid b-oxidation pathway. In mammalian tissue, many straight-chain acyl-CoA dehydrogenases possess different substrate specificities. In rare cases, irregularities in medium-chain acyl-CoA dehydrogenase can cause fasting hypoglycemia, hepatic dysfunction and encephalopathy, often resulting in death in infancy. MCAD, also designated acyl-CoA dehydrogenase, medium-chain (ACADM) and MCADH, is a homotetramer. The MCAD gene encodes a 421 amino acid protein with characteristics of mitochondrial protein transit peptides. The protein shows 88% sequence identity with MCAD of pig origin. Medium-chain acyl-CoA dehydrogenase catalyzes the initial reaction in the b-oxidation of C4 to C12 straight-chain acyl-CoAs. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MCAD of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 196-375 mapping within an internal region of MCAD of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 196-375 mapping within an internal region of MCAD of human origin |
| Entrez Gene | 34 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lower stomach tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of MCAD expression in non-transfected 293T: (A), human MCAD transfected 293T: (B) and HeLa (C) whole cell lysates.| Application Notes | recommended for detection of MCAD of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
