Application
Western blot analysis of Dynein HC expression in IMR-32 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of cells in ductus seminiferus at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Dynein HC expression in L8 (A) and LNCaP (B) whole cell lysates.| Product Name | Dynein HC Antibody (R-325) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 4320-4644 of Dynein heavy chain of rat origin |
| Modification Notes | Dyneins are multisubunit, high molecular weight ATPases that interact with microtubules to generate force by converting the chemical energy of ATP into the mechanical energy of movement. Cytoplasmic or axonemal Dynein heavy, intermediate, light and light-intermediate chains are all components of minus end-directed motors; the complex transports cellular cargos towards the central region of the cell. Axonemal Dynein motors contain one to three non-identical heavy chains and cause a sliding of microtubules in the axonemes of cilia and flagella in a mechanism necessary for cilia to beat and propel the cell. Cytoplasmic Dynein is an approximately 12 subunit complex of two heavy chains, two intermediate chains to anchor Dynein to its cargo, four smaller intermediate chains and several light chains. It performs functions necessary for cell survival such as organelle transport and centrosome assembly. The carboxy terminus of Dynein is important for microtubule-dependent motility and is highly conserved, while the amino terminal regions are more variable. Several proteins regulate Dynein activity, including dynactin, LIS1 and NudEL(NudE-like). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Dynein heavy chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 4320-4644 of Dynein heavy chain of rat origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 4320-4644 of Dynein heavy chain of rat origin |
| Entrez Gene | 1778 |
|---|
Application
Western blot analysis of Dynein HC expression in IMR-32 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of cells in ductus seminiferus at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Dynein HC expression in L8 (A) and LNCaP (B) whole cell lysates.| Application Notes | recommended for detection of Dynein heavy chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
