Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoskeletal staining of myocytes.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.Application
Western blot analysis of dystrophin expression in L8 (A) and A-10 (B) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded smooth muscle tissue showing membrane and cytoplasmic localization.| Product Name | dystrophin Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 801-1100 mapping within an internal region of dystrophin of human origin |
| Modification Notes | Dystrophin-glycoprotein complex (DGC) connects the F-Actin cytoskeleton on the inner surface of muscle fibers to the surrounding extracellular matrix, through the cell membrane interface. A deficiency in this protein contributes to Duchenne (DMD) and Becker (BMD) muscular dystrophies. The human dystrophin gene measures 2.4 megabases, has more than 80 exons, produces a 14 kb mRNA and contains at least eight independent tissue-specific promoters and two poly A sites. The dystrophin mRNA can undergo differential splicing and produce a range of transcripts that encode a large set of proteins. Dystrophin represents approximately 0.002% of total striated muscle protein and localizes to triadic junctions in skeletal muscle, where it is thought to influence calcium ion homeostasis and force transmission. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of dystrophin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 801-1100 mapping within an internal region of dystrophin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 801-1100 mapping within an internal region of dystrophin of human origin |
| Entrez Gene | 1756 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoskeletal staining of myocytes.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.
Application
Western blot analysis of dystrophin expression in L8 (A) and A-10 (B) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded smooth muscle tissue showing membrane and cytoplasmic localization.| Application Notes | recommended for detection of dystrophin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
