Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing membrane staining of red and white pulp cells.Application
Western blot analysis of CD4 expression in non-transfected: (A) and human CD4 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CD4 expression in CCRF-CEM whole cell lysate.| Product Name | CD4 Antibody (H-370) FITC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 26-396 of CD4 of human origin |
| Modification Notes | The T cell receptor (TCR) is a heterodimer composed of either å and ∫ or © and ∂ chains. CD3 chains and the CD4 or CD8 co-receptors are also required for efficient signal transduction through the TCR. The TCR is expressed on T helper and T cytotoxic cells that can be distinguished by their expression of CD4 and CD8; T helper cells express CD4 proteins and T cytotoxic cells display CD8. CD4 is also expressed on cortical cells, mature medullary thymocytes, microglial cells and dendritic cells. CD4 (also designated T4 and Leu 3), is a membrane glycoprotein that contains four extracellular immunoglobin-like domains. The TCR in association with CD4 can bind class II MHC molecules presented by the antigen-presenting cells. The CD4 protein functions by increasing the avidity of the interaction between the TCR and an antigen-class II MHC complex. An additional role of CD4 is to function as a receptor for HIV. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CD4 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 26-396 of CD4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 26-396 of CD4 of human origin |
| Entrez Gene | 920 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing membrane staining of red and white pulp cells.
Application
Western blot analysis of CD4 expression in non-transfected: (A) and human CD4 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CD4 expression in CCRF-CEM whole cell lysate.| Application Notes | recommended for detection of CD4 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
