Application
Immunofluorescence staining of methanol-fixed JEG-3 cells showing membrane localization.Application
Western blot analysis of GABAA Rε expression in JAR (A) and Hep G2 (B) whole cell lysates.| Product Name | GABAA Rε Antibody (H-110) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-110 mapping at the N-terminus of GABAA Rε of human origin |
| Modification Notes | GAD-65 and GAD-67, glutamate decarboxylases, function to catalyze the production of GABA (gamma-aminobutyric acid). In the central nervous system GABA functions as the main inhibitory transmitter by increasing a Cl- conductance that inhibits neuronal firing. GABA has been shown to activate both ionotropic (GABAA) and metabotropic (GABAB) receptors as well as a third class of receptors called GABAC. Both GABAA and GABAC are ligand-gated ion channels, however, they are structurally and functionally distinct. Members of the GABAA receptor family include GABAA Rå1-6, GABAA R ∫1-3, GABAA R©1-3, GABAA R∂, GABAA Ré, GABAA Rρ1 and GABAA Rρ2. The GABAB family is composed of GABAB R1å and GABAB R1∫. GABA transporters have also been identified and include GABA T-1, GABA T-2 and GABA T-3 (also designated GAT-1, -2, and -3). The GABA transporters function to terminate GABA action. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GABAA Rε of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-110 mapping at the N-terminus of GABAA R&epsilon of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-110 mapping at the N-terminus of GABAA Rε of human origin |
| Entrez Gene | 2564 |
|---|
Application
Immunofluorescence staining of methanol-fixed JEG-3 cells showing membrane localization.
Application
Western blot analysis of GABAA Rε expression in JAR (A) and Hep G2 (B) whole cell lysates.| Application Notes | recommended for detection of GABAA Rε of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
