Application
Western blot analysis of phosphorylated p53 expression in untreated (A) and hydroxyurea-treated (B) HT-29 cell extracts.Application
Western blot analysis of p53 phosphorylation in non-transfected: (A) and human p53 transfected: (B) 293T whole cell lysates.| Product Name | p-p53 Antibody (Thr 18) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Thr 18 phosphorylated p53 of human origin by WB and IP |
| Modification | Phosphoralated |
| Modification Notes | p53 is a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor that upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation and cell cycle control mechanisms. p53 localizes to the nucleus yet can be chaperoned to the cytoplasm by the negative regulator MDM2, an E3 ubiquitin ligase that is upregulated in the presence of active p53, where MDM2 polyubiquitinates p53 for proteasome targeting. p53 can assemble into tetramers in the absence of DNA, fluctuates between latent and active (DNA-binding) conformations, and is differentially activated through posttranslational modifications including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) (amino acids 110-286) of p53 can compromise energetically favorable association with cis elements and are implicated in several human cancers. Phosphorylation of p53 at residue Thr 155 is mediated by the COP9 signalosome (CSN) and targets p53 to ubiquitin-26S Proteasome-dependent degradation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| See 1 citations for p-p53Antibody (Thr 18): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Thr 18 phosphorylated p53 of human origin by WB and IP |
|---|---|
| Tissue Specificity | recommended for detection of Thr 18 phosphorylated p53 of human origin by WB and IP |
| Entrez Gene | 7157 |
|---|
Application
Western blot analysis of phosphorylated p53 expression in untreated (A) and hydroxyurea-treated (B) HT-29 cell extracts.
Application
Western blot analysis of p53 phosphorylation in non-transfected: (A) and human p53 transfected: (B) 293T whole cell lysates.| Application Notes | See 1 citations for p-p53Antibody (Thr 18): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
