Application
Western blot analysis of CHCHD4 expression in non-transfected: (A) and mouse CHCHD4 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CHCHD4 expression in non-transfected: (A) and human CHCHD4 transfected: (B) 293T whole cell lysates.| Product Name | CHCHD4 Antibody (H-107) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-107 mapping at the N-terminus of CHCHD4 of human origin |
| Modification Notes | CHCHD4 (coiled-coil-helix-coiled-coil-helix domain containing 4), also known as MIA40, is an 142 amino acid protein that contains one CHCH domain and localizes to the mitochondrial intermembrane space. Expressed in a variety of tissues with particularly high expression in kidney and liver, CHCHD4 exists as a homooligomer that is required for the folding of small cysteine-containing proteins. Specifically, CHCHD4 is thought to function via a disulfide relay system that oxidizes precursor proteins, thereby promoting their folding within mitochondria. CHCHD4 is expressed as multiple alternatively spliced isoforms that are encoded by a gene which maps to human chromosome 3. Chromosome 3 is made up of about 214 million bases encoding over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CHCHD4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-107 mapping at the N-terminus of CHCHD4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-107 mapping at the N-terminus of CHCHD4 of human origin |
| Entrez Gene | 131474 |
|---|
Application
Western blot analysis of CHCHD4 expression in non-transfected: (A) and mouse CHCHD4 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CHCHD4 expression in non-transfected: (A) and human CHCHD4 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of CHCHD4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
