Application
Western blot analysis of Rab 7 expression in non-transfected: (A) and mouse Rab 7 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and Rab 7 siRNA silenced HeLa (B) cells showing diminished nuclear staining in the siRNA silenced cells. Cells probed with Rab 7 (H-50): .Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.Application
Western blot analysis of Rab 7 expression in non-transfected control (A) and Rab 7 siRNA transfected (B) HeLa cells. Blot probed with Rab 7 (H-50): . emerin (H-12): used as specificity and loading control.Application
Western blot analysis of Rab 7 expression in NIH/3T3 (A) and A-431 (B) whole cell lysates.| Product Name | Rab 7 Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 158-207 mapping at the C-terminus of Rab 7 of human origin |
| Modification Notes | The Ras-related superfamily of guanine nucleotide binding proteins, which includes the Ral/Rec, Rap, R-Ras, and Rho/Rab subfamilies, exhibit 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the Sec4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. Several members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Rab 7 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 158-207 mapping at the C-terminus of Rab 7 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 158-207 mapping at the C-terminus of Rab 7 of human origin |
| Entrez Gene | 7879 |
|---|
Application
Western blot analysis of Rab 7 expression in non-transfected: (A) and mouse Rab 7 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and Rab 7 siRNA silenced HeLa (B) cells showing diminished nuclear staining in the siRNA silenced cells. Cells probed with Rab 7 (H-50): .
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.
Application
Western blot analysis of Rab 7 expression in non-transfected control (A) and Rab 7 siRNA transfected (B) HeLa cells. Blot probed with Rab 7 (H-50): . emerin (H-12): used as specificity and loading control.
Application
Western blot analysis of Rab 7 expression in NIH/3T3 (A) and A-431 (B) whole cell lysates.| Application Notes | recommended for detection of Rab 7 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
