Application
Western blot analysis of connexin 43 phosphorylation in non-transfected: (A) and human connexin 43 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of connexin 43 phosphorylation in non-transfected: (A) and mouse connexin 43 transfected: (B) 293T whole cell lysates.Application
Antibodies tested include connexin 43 (H-150): (A,B), p-connexin 43 (Ser279/282)-R: (C,D) and p-connexin 43 (Ser 255): (E,F).| Product Name | p-connexin 43 Antibody (Ser 255) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ser 255 phosphorylated connexin 43 of mouse and human origin by WB, IP, IF and ELISA |
| Modification | Phosphoralated |
| Modification Notes | The connexins are a group of gap junction proteins, which form a hexamer to compose a connexon. Clusters of connexons form a gap junction through which low molecular weight proteins may diffuse from cell to cell (1–3). Several mammalian cells with malignant phenotypes exhibit decreased connexin expression and gap junction communication (4). There is a decrease in gap junctional communication in src transformed cells. The decreased communication appears to be associated with tyrosine phospho-rylation of Connexin 43 (5). Activated c-Src phosphorylates the C-terminal tail of Connexin 43 on residue Tyr 265, resulting in a stable interaction between both proteins leading to inhibition of gap junctional communication (6). In addition to tyrosine phosphorylation, Connexin 43 has also been shown to be phos-phorylated on serine in the absence of src kinases and on both serine and tyrosine in cells expressing src kinases such as pp60v-src and/or c-src (7). In human vascular endothelial cells, Connexin 43 is postranslationally modified during mitosis, and mitosis-specific phosphorylation of Connexin 43 correlates with the transient loss of gap junction intercellular communication and redistribution of Connexin 43 (8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| blocking peptide, sc-12899 P: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 255 phosphorylated connexin 43 of mouse and human origin by WB IP IF and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Ser 255 phosphorylated connexin 43 of mouse and human origin by WB, IP, IF and ELISA |
| Entrez Gene | 14609 |
|---|
Application
Western blot analysis of connexin 43 phosphorylation in non-transfected: (A) and human connexin 43 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of connexin 43 phosphorylation in non-transfected: (A) and mouse connexin 43 transfected: (B) 293T whole cell lysates.
Application
Antibodies tested include connexin 43 (H-150): (A,B), p-connexin 43 (Ser279/282)-R: (C,D) and p-connexin 43 (Ser 255): (E,F).| Application Notes | blocking peptide, sc-12899 P: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
