Application
Western blot analysis of MAT Iα expression in non-transfected: (A) and human MAT Iα transfected: (B) 293 whole cell lysates.Application
Western blot analysis of MAT Iα expression in human fetal liver tissue extract.| Product Name | MAT Iα Antibody (Z-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of MAT Iα of human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Modification Notes | Methionine adenosyltransferase (MAT) catalyzes the formation of S-adenosyltransferase (AdoMet) for methionine catabolism in the liver. Two different genes, MAT1A and MAT2A, encode a liver specific and non-liver specific form of MAT, designated MAT Iå and MAT IIå, respectively. Inactivation of the liver specific gene product, designated MAT I/III, associates with liver diseases such as cirrhosis. MAT Iå expression also correlates with a differentiated phenotype, whereas liver cells expressing MAT IIå present a dedifferentiated phenotype and lowered AdoMet synthesis. Likewise, NF-kB and TNFå cause a switch from MAT Iå to MAT IIå expression in human hepatocellular carcinoma (HCC), which facilitates cancer cell growth. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of MAT I&alpha of human origin by WB IP and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of MAT Iα of human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 4143 |
|---|
Application
Western blot analysis of MAT Iα expression in non-transfected: (A) and human MAT Iα transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of MAT Iα expression in human fetal liver tissue extract.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
