Application
Western blot analysis of HERP expression in SK-N-MC whole cell lysate.Application
Western blot analysis of HERP expression in non-transfected: (A) and human HERP transfected: (B) 293T whole cell lysates.| Product Name | HERP Antibody (H-59) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 125-183 mapping within an internal region of HERP of human origin |
| Modification Notes | The endoplasmic reticulum (ER) stress response is triggered by the accumulation of unfolded proteins within the ER and is characterized by three events: the inhibition of translation (to prevent further protein accumulation), the up-regulated expression of polypeptide-folding proteins (known as the unfolded protein response, or UPR) and the degradation of misfolded proteins by the ER-associated protein degradation (ERAD) system. HERP, also known as HERPUD1 (homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1), SUP or MIF1, is a 391 amino acid multi-pass membrane protein that localizes to the ER and contains one N-terminal ubiquitin-like domain. Widely expressed with highest expression in the brain, HERP is a component of the ERAD system and, via its ubiquitin-like domain, is thought to be involved in the destruction of misfolded proteins. Three isoforms of HERP exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HERP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 125-183 mapping within an internal region of HERP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 125-183 mapping within an internal region of HERP of human origin |
| Entrez Gene | 9709 |
|---|
Application
Western blot analysis of HERP expression in SK-N-MC whole cell lysate.
Application
Western blot analysis of HERP expression in non-transfected: (A) and human HERP transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of HERP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
