Application
Western blot analysis of Aph-1 expression in non-transfected 293T: (A), mouse Aph-1 transfected 293T: (B) and HeLa (C) whole cell lysates.| Product Name | Aph-1 Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 181-230 mapping near the C-terminus of Aph-1 of human origin |
| Modification Notes | Four proteins comprise the ©-secretase complex: presenilin, nicastrin, Aph-1, and PEN-2. Together, these proteins mediate cell surface signaling pathways for a variety of type I membrane proteins, notably amyloid-beta precursor protein, a protein implicated in the development of Alzheimer’s disease, via intramembrane proteolysis. The proteins assemble into a proteolytically active complex in the Golgi/trans-Golgi network (TGN) compartments. Assembly leads to autocleavage of presenilin into two subunits to create the active site of ©-secretase, an important step in understanding the mechanisms involved in the etiology and possible treatment of Alzheimer’s disease. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Aph-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 181-230 mapping near the C-terminus of Aph-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 181-230 mapping near the C-terminus of Aph-1 of human origin |
| Entrez Gene | 51107 |
|---|
Application
Western blot analysis of Aph-1 expression in non-transfected 293T: (A), mouse Aph-1 transfected 293T: (B) and HeLa (C) whole cell lysates.| Application Notes | recommended for detection of Aph-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
