Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal cells.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human brain tissue showing membrane and cytoplasmic staining.Application
Western blot analysis of PLC β1 expression in HeLa whole cell lysate (A) and mouse brain tissue extract (B).| Product Name | PLC β1 Antibody (R-233) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 831-1063 mapping within an internal region of PLC β1 of rat origin |
| Modification Notes | Phosphoinositide-specific phospholipase C (PLC) plays a critical role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1, 4, 5-triphosphate and diacylglycerol from phosphatidylinositol 4, 5 bisphosphate. There are many mammalian PLC isozymes, including PLC ∫1, PLC ∫2, PLC ∫3, PLC ∫4, PLC ©1, PLC ©2, PLC ∂1 and PLC ∂2 and PLCe. PLC ∫1, one of the PLC ∫ isozymes, exists as two immunologically distinguishable proteins (PLC-∫1a) and (PLC ∫1b). The two isoforms encode in two distinct transcripts and are generated by alternative splicing of a single gene. PLC ∫1a is preferentially expressed in the cytosol, whereas PLC ∫1b is predominantly localized in the nuclei. PLC ∫1 is a G protein-dependent phosphodiesterase that hydrolyses phosphatidylinositol 4,5 biphosphate into inositol 1,4,5-triphosphate and diacylglycerol after the stimulation of a variety of neurotransmitter receptors at the cell surface. The C-terminal region of PLC ∫1 has Gq GAP activity and has ability to interact with Gq and other PLC-∫1 molecules. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PLC β1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 831-1063 mapping within an internal region of PLC &beta 1 of rat origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 831-1063 mapping within an internal region of PLC β1 of rat origin |
| Entrez Gene | 23236 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal cells.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human brain tissue showing membrane and cytoplasmic staining.
Application
Western blot analysis of PLC β1 expression in HeLa whole cell lysate (A) and mouse brain tissue extract (B).| Application Notes | recommended for detection of PLC β1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
