Application
Western blot analysis of KLHL2/3 expression in mouse testis tissue extract.| Product Name | KLHL2/3 Antibody (H-253) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 315-565 mapping near the C-terminus of KLHL2 of human origin |
| Modification Notes | KLHL2 (kelch-like protein 2), also known as actin-binding protein Mayven, is a 593 amino acid actin-binding protein. Highly expressed in brain, KLHL2 is thought to play a role in the organization of the actin cytoskeleton in brain cells. KLHL3 (kelch-like protein 3), is a 587 amino acid protein active in the protein ubiquitination pathway. KLHL3 is thought to be a substrate-specific adapter of an E3 ubiquitin-ligase complex that mediates the ubiquitination of target proteins, which leads to proteasomal degradation of the target protein. KLHL2 and KLHL3 proteins contain six kelch repeats and one BTB (POZ) domain. The BTB (Broad-Complex, Tramtrack and Bric a brac) domain, also known as the POZ (Poxvirus and Zinc finger) domain, is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. Proteins that contain BTB domains are thought to be involved in transcriptional regulation via control of chromatin structure and function. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of KLHL2 and KLHL3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 315-565 mapping near the C-terminus of KLHL2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 315-565 mapping near the C-terminus of KLHL2 of human origin |
| Entrez Gene | 11275 |
|---|
Application
Western blot analysis of KLHL2/3 expression in mouse testis tissue extract.| Application Notes | recommended for detection of KLHL2 and KLHL3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
