Application
Western blot analysis of POFUT1 expression in non-transfected: (A) and mouse POFUT1 transfected: (B) 293T whole cell lysates.| Product Name | POFUT1 Antibody (H-280) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 21-300 mapping near the N-terminus of POFUT1 of human origin |
| Modification Notes | Glycosyltransferases that mediate the regio- and stereoselective transfer of sugars, such as the fucosyltransferases, determine cell surface-carbohydrate profiles, which is an essential interface for biological recognition processes. Fucosyltransferases catalyze the covalent association of fucose to different positional linkages in sugar acceptor molecules. POFUT1 (protein O-fucosyltransferase 1), also known as FUT12, O-FUT or O-FucT-1, is a 388 amino acid protein that localizes to the endoplasmic reticulum and belongs to the fucosyltransferase subfamily of glycosyltransferases. Highly expressed in pancreas, kidney, lung, heart, brain, liver, placenta and skeletal muscle, POFUT1 uses manganese to catalyze the attachment (specifically the O-glycosidic linkage) of fucose to a conserved serine or threonine residue on a protein acceptor. Via its cataytic activity, POFUT1 plays an important role in Notch signaling, as Notch ligands can serve as POFUT1 substrates. Two isoforms of POFUT1 exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of POFUT1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 21-300 mapping near the N-terminus of POFUT1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 21-300 mapping near the N-terminus of POFUT1 of human origin |
| Entrez Gene | 23509 |
|---|
Application
Western blot analysis of POFUT1 expression in non-transfected: (A) and mouse POFUT1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of POFUT1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
