Application
Antibodies tested include p-MEK-2 (Thr 394): (A, B, C) and MEK-2 (C-16): (D, E, F).Application
Western blot analysis of MEK-2 phosphorylation in untreated (A), SS and EGF treated (B) and SS, EGF and lambda protein phosphatase treated (C) HEK293 whole cell lysates.Application
Blots were probed with p-MEK-2 (Thr 394): preincubated with its cognate phosphorylated peptide (A,B) and p-MEK-2 (Thr 394): (C).Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic and membrane staining.| Product Name | p-MEK-2 Antibody (Thr 394) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Thr 394 of MEK-2 of human origin |
| Modification | Phosphoralated |
| Modification Notes | A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Thr 394 phosphorylated MEK-2 of human and rat origin and correspondingly phosphorylated Thr 395 of mouse origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Thr 394 of MEK-2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Thr 394 of MEK-2 of human origin |
| Entrez Gene | 5605 |
|---|
Application
Antibodies tested include p-MEK-2 (Thr 394): (A, B, C) and MEK-2 (C-16): (D, E, F).
Application
Western blot analysis of MEK-2 phosphorylation in untreated (A), SS and EGF treated (B) and SS, EGF and lambda protein phosphatase treated (C) HEK293 whole cell lysates.
Application
Blots were probed with p-MEK-2 (Thr 394): preincubated with its cognate phosphorylated peptide (A,B) and p-MEK-2 (Thr 394): (C).
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic and membrane staining.| Application Notes | recommended for detection of Thr 394 phosphorylated MEK-2 of human and rat origin and correspondingly phosphorylated Thr 395 of mouse origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
