Application
Western blot analysis of PA28γ expression in Hep G2 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic localization.| Product Name | PA28γ Antibody (Z-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of PA28γ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Modification Notes | PA28 is an interferong (IFNg) inducible proteasome activator required for presentation of certain major histocom-patibility (MHC) class I antigens. PA28©, for Proteasome activator 28©, is also known as REG-gamma, Ki nuclear autoantigen and Proteasome Activator Complex Subunit 3. PA28å and PA28∫ co-localize in the cell, whereas PA28© has a unique distribution. A functional relationship between PA28© and the å and ∫ PA28 proteins is unknown. PA28© complexed with the proteasome may serve a function other than or in addition to activation, but PA28© may also have a proteasome-independent function. The PA28 complex is expressed constitutively in antigen-presenting cells. Downregulation of PA28 results in abnormal proteasome activation and has been implicated in the development of intimal hyperplasia in animal models. The PMSE3 gene maps to chromosome 17q12-q21 and encodes the g-subunit of the proteasome activator PA28. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of PA28&gamma of mouse rat and human origin by WB IP IF IHC(P) and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of PA28γ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 10197 |
|---|
Application
Western blot analysis of PA28γ expression in Hep G2 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
