Application
Western blot analysis of mEH expression in Hep G2 whole cell lysate.| Product Name | mEH Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 156-455 mapping at the C-terminus of mEH of human origin |
| Modification Notes | Epoxide hydrolases (EHs) are biotransformation enzymes that catalyze the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water. The enzymatic hydration is essentially irreversible and produces mainly metabolites of lower reactivity that can be conjugated and excreted, and, therefore, are generally regarded as detoxifying. Microsomal EH (mEH) is one of many enzymes involved in the metabolism of endogenous and exogenous toxicants such as tobacco-derived carcinogens. mEH exhibits a broad substrate specificity, while the soluble EH (sEH) is an enzyme with a "complementary" substrate specificity to mEH. The mEH protein is encoded by the EPHX1 gene, which maps to chromosome 1q42.12. Polymorphism of the EPHX1 gene is a risk factor ovarian cancer and hepatocellular carcinoma. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of mEH of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 156-455 mapping at the C-terminus of mEH of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 156-455 mapping at the C-terminus of mEH of human origin |
| Entrez Gene | 2052 |
|---|
Application
Western blot analysis of mEH expression in Hep G2 whole cell lysate.| Application Notes | recommended for detection of mEH of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
