Application
Western blot analysis of TIS11B expression in JAR (A), Ramos (B), A549 (C), MCR7 (D) and SW480 (E) whole cell lysates.Application
Western blot analysis of TIS11B expression in K-562 (A) and THP-1 (B) nuclear extracts.Application
Western blot analysis of TIS11B expression in HeLa (A), K-562 (B), THP-1 (C), A549 (D), KNRK (E) and WI 38 (F) nuclear extracts.Application
Western blot analysis of TIS11B expression in JAR (A), Ramos (B), A549 (C) and HT-1080 (D) whole cell lysates.Application
Western blot analysis of TIS11B expression in human placenta tissue extract.Application
Western blot analysis of TIS11B expression in HeLa whole cell lysate.| Product Name | TIS11B Antibody (A-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of TIS11B of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Modification Notes | TIS11B (tetradecanoyl phorbol acetate-inducible-sequence 11b), also known as ZFP36L1, BRF1 (butyrate response factor 1), ERF1 (EGF-response factor 1), cMG1, Berg36 or RNF162B, is a member of the tristetraprolin family. Tristetraprolin (TTP), or TIS11, is a zinc-binding protein encoded by the immediate-early response gene, Zfp-36. TIS11B, a relative of TTP, localizes to the nucleus and may function as a transcription factor involved in regulating the growth factor response. It is an evolutionarily conserved protein containing two C3H1-type zinc fingers and a repeating cys-his motif. TIS11B is an mRNA binding protein and is known to interact with the 3’-untranslated region of VEGF mRNA, thereby decreasing its stability. This suggests that TIS11B is a potential target in antiangiogenic therapy. In addition, TIS11B may also be an important regulator of myogenesis, as its expression is upregulated during murine myoblast differentiation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of TIS11B of mouse rat and human origin by WB IP and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of TIS11B of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 677 |
|---|
Application
Western blot analysis of TIS11B expression in JAR (A), Ramos (B), A549 (C), MCR7 (D) and SW480 (E) whole cell lysates.
Application
Western blot analysis of TIS11B expression in K-562 (A) and THP-1 (B) nuclear extracts.
Application
Western blot analysis of TIS11B expression in HeLa (A), K-562 (B), THP-1 (C), A549 (D), KNRK (E) and WI 38 (F) nuclear extracts.
Application
Western blot analysis of TIS11B expression in JAR (A), Ramos (B), A549 (C) and HT-1080 (D) whole cell lysates.
Application
Western blot analysis of TIS11B expression in human placenta tissue extract.
Application
Western blot analysis of TIS11B expression in HeLa whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
