Application
Western blot analysis of CA II expression in non-transfected: (A) and human CA II transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CA II expression in non-transfected 293T: (A), mouse CA II transfected 293T: (B) and HEL 92.1.7 (C) whole cell lysates.Application
Western blot analysis of CA II expression in non-transfected: (A) and human CA II transfected: (B) 293T whole cell lysates and mouse kidney tissue extract (C).Application
Western blot analysis of CA II expression in Caki-1 (A) and Hel 92.1.7 (B) whole cell lysates and rat kidney tissue extract (C).Application
Immunofluorescence staining of methanol-fixed K-562 cells showing cytoplasmic localization.Application
Western blot analysis of CA II expression in mouse kidney tissue extract.Application
Western blot analysis of CA II expression in c4 whole cell lysate.| Product Name | CA II Antibody (H-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 191-260 mapping at the C-terminus of CA II of human origin |
| Modification Notes | Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes including respiration, calcification, acid-base balance and bone resorption, as well as the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. They show extensive diversity in distribution and in their subcellular localization. The human CA2 gene, which maps to chromosome 8q21, encodes CA II, a cytoplasmic protein that has the highest turnover rate and widest tissue distribution of any known human CA isozyme. The human CA4 gene, which maps to chromosome 17q23, encodes CA IV, a membrane-anchored isozyme that is expressed on the luminal surfaces of pulmonary capillaries and proximal renal tubules. The human CA9, CA12 and CA14 genes, which map to chromosomes 9p13, 15q22 and 1q21, respectively, encode transmembrane proteins that have unique patterns of tissue-specific expression. CA IX is specifically expressed in clear-cell renal carcinomas, whereas CA XII is highly expressed in normal tissues, such as kidney, colon and pancreas. Human CA XIV is also expressed in normal tissues, such as brain, but differs from CA XII in its expression pattern. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CA II of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 191-260 mapping at the C-terminus of CA II of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 191-260 mapping at the C-terminus of CA II of human origin |
| Entrez Gene | 760 |
|---|
Application
Western blot analysis of CA II expression in non-transfected: (A) and human CA II transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CA II expression in non-transfected 293T: (A), mouse CA II transfected 293T: (B) and HEL 92.1.7 (C) whole cell lysates.
Application
Western blot analysis of CA II expression in non-transfected: (A) and human CA II transfected: (B) 293T whole cell lysates and mouse kidney tissue extract (C).
Application
Western blot analysis of CA II expression in Caki-1 (A) and Hel 92.1.7 (B) whole cell lysates and rat kidney tissue extract (C).
Application
Immunofluorescence staining of methanol-fixed K-562 cells showing cytoplasmic localization.
Application
Western blot analysis of CA II expression in mouse kidney tissue extract.
Application
Western blot analysis of CA II expression in c4 whole cell lysate.| Application Notes | recommended for detection of CA II of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
