Application
Western blot analysis of HMGCL expression in Hep G2 whole cell lysate.Application
Western blot analysis of HMGCL expression in A-431 whole cell lysate.| Product Name | HMGCL Antibody (Y-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of HMGCL of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Modification Notes | HMGCL (3-hydroxy-3-methylglutaryl-Coenzyme A (CoA) lyase), also known as HMG-CoA lyase or HL, is a mitochondrial matrix protein that belongs to the HMG-CoA lyase family of proteins. Expressed in liver, lymphoblasts and fibroblasts, HMGCL exists as a homodimer and participates in leucine catabolism and ketogenesis, the hepatic synthesis of ketone bodies that, during fasting, provide a major source of energy for heart, brain and kidney. More specifically, HMGCL catalyzes the final step of these processes, the cleavage of 3-hydroxy-3-methylglutaryl CoA to acetoacetic acid and acetyl CoA. Mutations in the gene encoding HMGCL can lead to HMG-CoA lyase deficiency (also known as HL deficiency or hydroxymethylglutaricaciduria), a metabolic disease that, if left untreated, results in hypoglycemia and coma. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of HMGCL of mouse rat and human origin by WB IP and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of HMGCL of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 3155 |
|---|
Application
Western blot analysis of HMGCL expression in Hep G2 whole cell lysate.
Application
Western blot analysis of HMGCL expression in A-431 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
