Application
Western blot analysis of PARL expression in Jurkat whole cell lysate.Application
Western blot analysis of PARL expression in COLO 320DM whole cell lysate.| Product Name | PARL Antibody (A-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of PARL of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Modification Notes | Presenilins associated rhombiod-like protein (PARL) is a mitochondrial intramembrane-cleaving protease belonging to the S54 family of proteins. PARL is involved in intramembrane regulated proteolysis as its catalytic activity involves the cleaving of signaling proteins at intracellular membranes to release active fragments in signal transduction cascades. Using a triad of histidine, serine and asparagine, PARL cleaves type-1 transmembrane domains. PARL is a multi-pass membrane protein localizing to the inner and outer mitochondrial membranes, but it can also be detected in the nucleus following proteolytical processing of P-beta. PARL co-localizes with the presenilins PSEN1 and PSEN2, the familial Alzheimer disease products. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of PARL of mouse rat and human origin by WB IP IF IHC(P) and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of PARL of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 55486 |
|---|
Application
Western blot analysis of PARL expression in Jurkat whole cell lysate.
Application
Western blot analysis of PARL expression in COLO 320DM whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
