Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing granular cytoplasmic staining of cells in tubules.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of TH expression in rat adrenal gland (A) and mouse brain (B) tissue extracts and truncated human recombinant TH fusion protein (C).Application
Immunofluorescence staining of methanol-fixed PC-12 cells showing cytoplasmic localization.| Product Name | TH Antibody (H-196) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-196 (with a deletion at 31-61) of TH (tyrosine hydroxylase) of human origin |
| Modification Notes | The enzyme tyrosine hydroxylase (TH), also designated tyrosine 3-monooxygenase (TY3H), catalyzes the conversion of tyrosine to L-dopa, which is the rate limiting step in the biosynthesis of catecholamines such as dopamine, adrenalin and noradrenalin. TH is thought to play a role in the pathogenesis of Parkinson’s disease, which is associated with reduced dopamine levels. Two transcription factor binding sites in the proximal region of the TH gene, the TPA-responsive element (TRE) and the c-AMP responsive element (CRE), have been implicated in the complex regulation of the TH gene. TH is also known to be upregulated by the glia maturation factor (GMF), a Cdc 10/SWI6 motif-containing protein called V-1, and a variety of additional compounds. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of TH of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-196 (with a deletion at 31-61) of TH (tyrosine hydroxylase) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-196 (with a deletion at 31-61) of TH (tyrosine hydroxylase) of human origin |
| Entrez Gene | 7054 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing granular cytoplasmic staining of cells in tubules.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of TH expression in rat adrenal gland (A) and mouse brain (B) tissue extracts and truncated human recombinant TH fusion protein (C).
Application
Immunofluorescence staining of methanol-fixed PC-12 cells showing cytoplasmic localization.| Application Notes | recommended for detection of TH of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
