Application
Western blot analysis of EPLIN expression in non-transfected: (A) and human EPLIN transfected: (B) 293 whole cell lysates.Application
Western blot analysis of EPLIN expression in non-transfected 293T: (A), mouse EPLIN transfected 293T: (B) and JAR (C) whole cell lysates.Application
Western blot analysis of EPLIN expression in HeLa whole cell lysate.Application
Western blot analysis of EPLIN expression in human placenta tissue extract.| Product Name | EPLIN Antibody (X-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of EPLIN of mouse and human origin by WB, IP and ELISA |
| Modification Notes | Epithelial protein lost in neoplasm (EPLIN) is a cytoskeleton-associated protein characterized by the presence of a single centrally located lin-11, isl-1 and mec-3 (LIM) domain. It also contains at least two Actin-binding domains, in which the C-terminal domain binds more effectively than the N-terminal domain. By binding Actin monomers and filaments, EPLIN is involved in regulation of the Actin cytoskeleton by increasing the number and size of Actin stress fibers, delaying filament nucleation, reducing formation of branched filaments and bundling of Actin filaments. It also inhibits membrane ruffling and Actin filament depolymerization. EPLIN is strongly expressed in placenta, kidney, pancreas, prostate, ovary, spleen and heart, and to a lesser degree in lung, liver, brain, skeletal muscle, thymus, testis and intestine. It is expressed as two isoforms, EPLIN-alpha and EPLIN-beta. Downregulation of EPLIN-alpha expression may contribute to the motility of invasive tumor cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of EPLIN of mouse and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of EPLIN of mouse and human origin by WB, IP and ELISA |
| Entrez Gene | 51474 |
|---|
Application
Western blot analysis of EPLIN expression in non-transfected: (A) and human EPLIN transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of EPLIN expression in non-transfected 293T: (A), mouse EPLIN transfected 293T: (B) and JAR (C) whole cell lysates.
Application
Western blot analysis of EPLIN expression in HeLa whole cell lysate.
Application
Western blot analysis of EPLIN expression in human placenta tissue extract.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
