Application
Western blot analysis of Oct-6 expression in HL-60 whole cell lysate.Application
Western blot analysis of Oct-6 expression in Jurkat whole cell lysate.| Product Name | Oct-6 Antibody (W-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Oct-6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Modification Notes | POU homeodomain proteins are transcriptional regulators that function in various developmental processes (e.g. cell division, differentiation, specification and survival of specific cell types) and participate in the determination of cell fate. The POU transcription factor Oct-6 (also designated SCIP and Tst-1) is expressed by late embryonic Schwann cells of the peripheral nervous system and is also expressed by nonmyelinating Schwann cells in adults. Oct-6 is strongly upregulated in promyelin cells because it is required for the timely differentiation of promyelin cells into myelinating cells. Oct-6 functions during myelination and is required for the proper downregulation of its own gene when myelination proceeds. c-Myc can act synergistically with the POU domain of Oct-6 to produce myelin disease pathogenesis in the mammalian central nervous system. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Oct-6 of mouse rat and human origin by WB IP IF IHC(P) and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of Oct-6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 5453 |
|---|
Application
Western blot analysis of Oct-6 expression in HL-60 whole cell lysate.
Application
Western blot analysis of Oct-6 expression in Jurkat whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
